Genetic and DNA Methylation Changes in Cotton (Gossypium) Genotypes and Tissues

Kenji Osabe; Jenny D. Clement; Frank Bedon; Filomena A. Pettolino; Lisa Ziolkowski; Danny J. Llewellyn; E. Jean Finnegan; Iain W. Wilson

doi:10.1371/journal.pone.0086049

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Open Access

Peer-reviewed

Research Article

Genetic and DNA Methylation Changes in Cotton (Gossypium) Genotypes and Tissues

Kenji Osabe,

Affiliation CSIRO, Plant Industry, ACT, Australia
⨯
Jenny D. Clement,

Affiliation CSIRO, Plant Industry, ACT, Australia
⨯
Frank Bedon,

Affiliation CSIRO, Plant Industry, ACT, Australia
⨯
Filomena A. Pettolino,

Affiliation CSIRO, Plant Industry, ACT, Australia
⨯
Lisa Ziolkowski,

Affiliation CSIRO, Plant Industry, ACT, Australia
⨯
Danny J. Llewellyn,

Affiliation CSIRO, Plant Industry, ACT, Australia
⨯
E. Jean Finnegan,

Affiliation CSIRO, Plant Industry, ACT, Australia
⨯
Iain W. Wilson

* E-mail: Iain.Wilson@csiro.au

Affiliation CSIRO, Plant Industry, ACT, Australia
⨯

Genetic and DNA Methylation Changes in Cotton (Gossypium) Genotypes and Tissues

Kenji Osabe,
Jenny D. Clement,
Frank Bedon,
Filomena A. Pettolino,
Lisa Ziolkowski,
Danny J. Llewellyn,
E. Jean Finnegan,
Iain W. Wilson

Published: January 20, 2014
https://doi.org/10.1371/journal.pone.0086049

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methylation sensitivity of BsiSI

Posted by BenSchulz on 29 Jan 2014 at 14:26 GMT

Do you have any reference for the complete methylation insensitivity of BsiSI?

According to the Restriction Enzyme Database (http://rebase.neb.com/) BsiSI does not cut if the two external cytosines of the CCGG-site are methylated (e.g. CHG-methylation at both strands). If BsiSI is actually methylation sensitive it is not suited to assess genetic diversity.

No competing interests declared.

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Genetic and DNA Methylation Changes in Cotton (Gossypium) Genotypes and Tissues

Genetic and DNA Methylation Changes in Cotton (Gossypium) Genotypes and Tissues

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methylation sensitivity of BsiSI

Posted by BenSchulz on 29 Jan 2014 at 14:26 GMT