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LA taq DNA polymerase has nuclease activity?

Posted by EmmanuelCorse on 16 Jan 2014 at 14:20 GMT

Dear author,

I read that PCR clamping with PNA may be use with a taq polymerase with no nuclease activity. But I see that LA taq has 3'-5' nuclease activity.

However, it seems that it work well!

Do you think that we can generalize, i.e. PNA work even using polymerase with nuclease activity?

No competing interests declared.