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closeSome questions to this paper2
Posted by Frank2013 on 14 Oct 2013 at 09:19 GMT
4, In Figure 4 A, the CD68 staining of WT mice.
I had no experience of this antibody, but from some colleagues they told me in WT mice there could be more positive cells.
And, in B, the small frame is an area with a plaque, or not? Certainly around a plaque there are a great amount of microglia.
5, In Figure6, Band D, you showed 12 sessions, and in the article, page3, Radial Arm Maze, you mentioned the test was for 24days twice a day.
This is a big confusion for me. If it was finished in 24 days, and twice a day, then there should be 48 sessions; or maybe 2 days per session with 12 sessions in total, then this sound reasonable for 24 days.
RE: Some questions to this paper2
bvissel replied to Frank2013 on 08 Nov 2013 at 00:45 GMT
Response from Amanda Wright and Bryce Vissel for questions 4-5.
Thank you for your questions regarding editorial points in our publication. Please find our answers below.
4. Question “In Figure 4 A, the CD68 staining of WT mice. I had no experience of this antibody, but from some colleagues they told me in WT mice there could be more positive cells. And, in B, the small frame is an area with a plaque, or not? Certainly around a plaque there are a great amount of microglia.”
CD68 is known to predominantly label activated microglia/macrophages. In this study, we quantified CD68 staining throughout the stratum radiatum and stratum lacunosum moleculare. Other publications (e.g. Kristic et al. J Neuroinflammation 2012 9:151) have shown similar levels of staining in WT mice to our study. In our study we have accurately quantified positive in WT mice and hAPP-J20 mice as indicated in figure 4.
Morphological changes and congregation of CD68 positive microglia occurred in hAPP-J20 mice, which presumably occurs around plaques, though double staining for plaques and CD68-positive microglia would need to be conducted in order to confirm this.
5, Question “In Figure 6, Band D, you showed 12 sessions, and in the article, page 3, Radial Arm Maze, you mentioned the test was for 24 days twice a day. This is a big confusion for me. If it was finished in 24 days, and twice a day, then there should be 48 sessions; or maybe 2 days per session with 12 sessions in total, then this sound reasonable for 24 days.”
The radial arm maze was conducted 2 times per day for 24 days. Data obtained over 2 days were pooled to form “Session”, as detailed in the Methods section of our manuscript