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Research Article

Analysis of Transcription Factor mRNAs in Identified Oxytocin and Vasopressin Magnocellular Neurons Isolated by Laser Capture Microdissection

  • Madison Humerick equal contributor,

    equal contributor Contributed equally to this work with: Madison Humerick, Jeffrey Hanson

    Affiliation: Laboratory of Neurochemistry, National Institute of Neurological Disease and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America

    Current address: West Virginia University School of Medicine, Morgantown, West Virginia, United States of America

    X
  • Jeffrey Hanson equal contributor,

    equal contributor Contributed equally to this work with: Madison Humerick, Jeffrey Hanson

    Affiliation: Laser Microdissection Core, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • Jaime Rodriguez-Canales,

    Affiliation: Laser Microdissection Core, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America

    Current address: Department of Translational Molecular Pathology, University of Texas - MD Anderson Cancer Center, Houston, Texas, United States of America

    X
  • Daniel Lubelski,

    Affiliation: Laboratory of Neurochemistry, National Institute of Neurological Disease and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America

    Current address: Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio, United States of America

    X
  • Omar M. Rashid,

    Affiliation: Laboratory of Neurochemistry, National Institute of Neurological Disease and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America

    Current address: New York Medical College, Valhalla, New York, United States of America

    X
  • Yasmmyn D. Salinas,

    Affiliation: Laboratory of Neurochemistry, National Institute of Neurological Disease and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America

    Current address: Yale School of Public Health, Yale University, New Haven, Connecticut, United States of America

    X
  • YiJun Shi,

    Affiliation: Laboratory of Neurochemistry, National Institute of Neurological Disease and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • Todd Ponzio,

    Affiliation: Laboratory of Neurochemistry, National Institute of Neurological Disease and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America

    Current address: Office of Technology Transfer, Naval Medical Research Center, Silver Spring, Maryland, United States of America

    X
  • Raymond Fields,

    Affiliation: Laboratory of Neurochemistry, National Institute of Neurological Disease and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • Michael R. Emmert-Buck,

    Affiliation: Laser Microdissection Core, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • Harold Gainer mail

    gainerh@ninds.nih.gov

    Affiliation: Laboratory of Neurochemistry, National Institute of Neurological Disease and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America

    X
  • Published: July 24, 2013
  • DOI: 10.1371/journal.pone.0069407

Reader Comments (2)

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RE: Response to query re preparation of slides for LCM

Posted by hgainer on 28 Aug 2013 at 17:42 GMT

My response is:

We do not wash the slides with water, & I don't recall any opaqueness that interfered with the LCM.
You could also consult the first author, M. Humerick via msteven8@mix.wvu.ed

We use the following protocol, the first wash is in 75% ethanol as follows:
1. Using the solutions prepared on the day of use, in a fume hood perform the following steps:
a. 75% Ethanol for 30 sec
b. 95% Ethanol for 5 sec
c. 100% Ethanol for 5 sec
d. 100% Ethanol for 5 sec
e. Xylene for 30 sec
f. Allow Xylene to completely evaporate
i. This usually takes 2-5 min.

The above is written in Table S2 in Supplements.

Good luck,
H. Gainer

No competing interests declared.