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Research Article

Evaluation of 16S rDNA-Based Community Profiling for Human Microbiome Research

  • Jumpstart Consortium Human Microbiome Project Data Generation Working Group mail

    bwb@broadinstitute.org

    Membership of the Jumpstart Consortium Human Microbiome Project Data Generation Working Group is provided in the Acknowledgments.

    X
  • Published: June 13, 2012
  • DOI: 10.1371/journal.pone.0039315
  • Featured in PLOS Collections

Reader Comments (2)

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poolingCalculator.xls

Posted by thackmann on 08 Aug 2013 at 17:48 GMT

This is a useful article that I enjoyed reading. Do you have a version of poolingCalculator.xls that you can post? This spreadsheet is referenced in Protocol S2, but I could not immediately find it here or at http://www.hmpdacc.org/.

No competing interests declared.

RE: poolingCalculator.xls

Doyle replied to thackmann on 15 Aug 2013 at 12:34 GMT

Unfortunately, I am unable to provide the document poolingCalcluator.xls. However, in section 3.5.1 of Protocol S2 you may find the formula implemented by the calculator. I've copied the relevant text below. I hope this is helpful. Best wishes.

-Doyle Ward

Using values from the SYBR Green or Quant-IT quantification, calculate pooling
amounts using the poolingCalculator.xls or according to the following formula:
Amount (uL) of each sample = ((vol/2)*(min)) / sampleconc
where:
Vol = total volume of each sample
Min = concentration in ng/ul of the sample with the lowest concentration
Sampleconc = concentration in ng/uL of target sample

Competing interests declared: Co-author of manuscript