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Research Article

Alternative Splicing and Extensive RNA Editing of Human TPH2 Transcripts

  • Maik Grohmann,

    Affiliations: Department of Human Molecular Genetics, Max Planck Institute for Molecular Genetics, Berlin, Germany, Department of Biology, Chemistry, and Pharmacy, Free University Berlin, Berlin, Germany, Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité-Universitätsmedizin, Berlin, Germany

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  • Paul Hammer,

    Affiliation: Department of Human Molecular Genetics, Max Planck Institute for Molecular Genetics, Berlin, Germany

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  • Maria Walther,

    Affiliation: Department of Human Molecular Genetics, Max Planck Institute for Molecular Genetics, Berlin, Germany

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  • Nils Paulmann,

    Affiliations: Department of Human Molecular Genetics, Max Planck Institute for Molecular Genetics, Berlin, Germany, Department of Biology, Chemistry, and Pharmacy, Free University Berlin, Berlin, Germany

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  • Andreas Büttner,

    Affiliation: Institute for Legal Medicine, Ludwig Maximilians University, Munich, Germany

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  • Wolfgang Eisenmenger,

    Affiliation: Institute for Legal Medicine, Ludwig Maximilians University, Munich, Germany

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  • Thomas C. Baghai,

    Affiliation: Department of Psychiatry, Ludwig Maximilians University, Munich, Germany

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  • Cornelius Schüle,

    Affiliation: Department of Psychiatry, Ludwig Maximilians University, Munich, Germany

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  • Rainer Rupprecht,

    Affiliation: Department of Psychiatry, Ludwig Maximilians University, Munich, Germany

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  • Michael Bader,

    Affiliation: Laboratory of Molecular Biology of Peptide Hormones, Max Delbrück Center for Molecular Medicine, Berlin, Germany

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  • Brigitta Bondy,

    Affiliation: Department of Psychiatry, Ludwig Maximilians University, Munich, Germany

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  • Peter Zill,

    Affiliation: Department of Psychiatry, Ludwig Maximilians University, Munich, Germany

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  • Josef Priller equal contributor,

    equal contributor Contributed equally to this work with: Josef Priller, Diego J. Walther

    Affiliation: Neuropsychiatry and Laboratory of Molecular Psychiatry, Charité-Universitätsmedizin, Berlin, Germany

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  • Diego J. Walther equal contributor mail

    equal contributor Contributed equally to this work with: Josef Priller, Diego J. Walther

    dwalther@molgen.mpg.de

    Affiliation: Department of Human Molecular Genetics, Max Planck Institute for Molecular Genetics, Berlin, Germany

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  • Published: January 29, 2010
  • DOI: 10.1371/journal.pone.0008956

Reader Comments (1)

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RNA editing patterns

Posted by baysalbe on 08 Aug 2013 at 15:50 GMT

I am wondering if authors have any explanation or confirmatory evidence for what appears to be allele-specific RNA editing in Table 1. In contrast to the A allele that shows RNA editing in TPH2a isoform in amygdala, the T allele of rs4290270 genomic SNP does not appear to show any editing.
The T allele could not be captured for the TPH2b isoform transcripts, the A allele of which shows abundant editing in all brain regions in Table 1. Were you able to see RNA editing of the T allele of TPH2b isoform by sequencing of the total RT-PCR product in T/T homozygous subjects?

Any comments on allele specific RNA editing is appreciated.

Thanks,

No competing interests declared.