Reader Comments
Post a new comment on this article
Post Your Discussion Comment
Please follow our guidelines for comments and review our competing interests policy. Comments that do not conform to our guidelines will be promptly removed and the user account disabled. The following must be avoided:
- Remarks that could be interpreted as allegations of misconduct
- Unsupported assertions or statements
- Inflammatory or insulting language
Thank You!
Thank you for taking the time to flag this posting; we review flagged postings on a regular basis.
closeFigure 7 Panel E
Posted by knudsonm on 23 Sep 2011 at 21:19 GMT
This is very interesting data. It is unclear why the 2DG + ABT had modest affects but the 2DG + ABT + D-glucose was more pronounced. One would think that the D-glucose would decrease the efficacy of 2DG, yet this is not what was seen. Can the authors comment on the rationale behind this triple combination??
RE: Figure 7 Panel E
rudy replied to knudsonm on 24 Sep 2011 at 00:10 GMT
Hi Dr. Knudson,
We thought about this a lot, but we do only have conjectures. Since in tissue cultre cells, 2DG in glucose-depleted medium did not prime cells for ABT-induced apoptosis (see Fig S2b), we though using p.i. where deoxyglucose enter areas in which there is no glucose, we added glucose just in case. There is an old 1953 article in which incubation of endothelial cells in media containing dexoyglucose and no glucose, it blocked all subsequent entry of glucose. They did not know why that was.
RE: RE: Figure 7 Panel E
knudsonm replied to rudy on 28 Sep 2011 at 14:19 GMT
Thanks Rudy- That is a very interesting idea. So are you suggesting that D-glucose is required so that the 2DG goes into the blood stream following IP injection? Also have you considered the possibility that the GLucose deprived cells don't activate caspases following 2DG becauce they are undergoing necrosis. Finally I looked for but could not find the reference from 1953 you mentioned. It is not cited in your paper- could you provide.
RE: RE: RE: Figure 7 Panel E
rudy replied to knudsonm on 30 Sep 2011 at 02:18 GMT
Hi Dr.Knudson,
It is possible that glucose depleted cells enter necrosis very quickly. But my HeLa cells were cultured in high glucose medium and they do well at least for a while, living on stored glycogen. For the record, 1953 article is actually 1956 article JBC 222, 671-6