Advertisement
Research Article

Neural Stem/Progenitor Cells from the Adult Human Spinal Cord Are Multipotent and Self-Renewing and Differentiate after Transplantation

  • Andrea J. Mothe mail,

    amothe@uhnres.utoronto.ca

    Affiliation: Division of Genetics and Development, Toronto Western Research Institute and Krembil Neuroscience Centre, Toronto Western Hospital, Toronto, Ontario, Canada

    X
  • Tasneem Zahir,

    Affiliation: Division of Genetics and Development, Toronto Western Research Institute and Krembil Neuroscience Centre, Toronto Western Hospital, Toronto, Ontario, Canada

    X
  • Carlo Santaguida,

    Affiliation: Division of Genetics and Development, Toronto Western Research Institute and Krembil Neuroscience Centre, Toronto Western Hospital, Toronto, Ontario, Canada

    X
  • Douglas Cook,

    Affiliation: Division of Genetics and Development, Toronto Western Research Institute and Krembil Neuroscience Centre, Toronto Western Hospital, Toronto, Ontario, Canada

    X
  • Charles H. Tator

    Affiliation: Division of Genetics and Development, Toronto Western Research Institute and Krembil Neuroscience Centre, Toronto Western Hospital, Toronto, Ontario, Canada

    X
  • Published: November 02, 2011
  • DOI: 10.1371/journal.pone.0027079

Reader Comments (2)

Post a new comment on this article

Staining of Neurospheres

Posted by Sameehan2301 on 25 Feb 2013 at 11:13 GMT

Thank you for a nicely written paper.
I just wanted to know how did you perform the staining of Neurospheres? Did you plate them on Poly-L-Lysine or embed them in OCT and sectioned them?
I am trying to stain Neurospheres in my experiment and the images are no where close to the ones which you show in the paper. So, was wondering whether I am missing a step or doing something wrong.?
Thank you.
Regards,
Sameehan Mahajani.

No competing interests declared.