Identifying mRNA, MicroRNA and Protein Profiles of Melanoma Exosomes

Deyi Xiao; Joanna Ohlendorf; Yinlu Chen; Douglas D. Taylor; Shesh N. Rai; Sabine Waigel; Wolfgang Zacharias; Hongying Hao; Kelly M. McMasters

doi:10.1371/journal.pone.0046874

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Research Article

Identifying mRNA, MicroRNA and Protein Profiles of Melanoma Exosomes

Deyi Xiao ,

Contributed equally to this work with: Deyi Xiao, Joanna Ohlendorf

Affiliation Department of Surgery, University of Louisville School of Medicine, Louisville, Kentucky, United States of America
⨯
Joanna Ohlendorf ,

Contributed equally to this work with: Deyi Xiao, Joanna Ohlendorf

Affiliation Department of Surgery, University of Louisville School of Medicine, Louisville, Kentucky, United States of America
⨯
Yinlu Chen,

Affiliation Microarray facility, University of Louisville School of Medicine, Louisville, Kentucky, United States of America
⨯
Douglas D. Taylor,

Affiliation Department of Obstetrics, Gynecology, and Women’s Health, University of Louisville School of Medicine, Louisville, Kentucky, United States of America
⨯
Shesh N. Rai,

Affiliation Department of Bioinformatics and Biostatistics, University of Louisville School of Medicine, Louisville, Kentucky, United States of America
⨯
Sabine Waigel,

Affiliation Microarray facility, University of Louisville School of Medicine, Louisville, Kentucky, United States of America
⨯
Wolfgang Zacharias,

Affiliations Microarray facility, University of Louisville School of Medicine, Louisville, Kentucky, United States of America, Department of Medicine and Department of Pharmacology and Toxicology, James Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, Kentucky, United States of America
⨯
Hongying Hao ,

* E-mail: h0hao001@louisville.edu (HH); mcmasters@louisville.edu (KMM)

Affiliation Department of Surgery, University of Louisville School of Medicine, Louisville, Kentucky, United States of America
⨯
Kelly M. McMasters

* E-mail: h0hao001@louisville.edu (HH); mcmasters@louisville.edu (KMM)

Affiliation Department of Surgery, University of Louisville School of Medicine, Louisville, Kentucky, United States of America
⨯

Identifying mRNA, MicroRNA and Protein Profiles of Melanoma Exosomes

Deyi Xiao,
Joanna Ohlendorf,
Yinlu Chen,
Douglas D. Taylor,
Shesh N. Rai,
Sabine Waigel,
Wolfgang Zacharias,
Hongying Hao,
Kelly M. McMasters

Published: October 9, 2012
https://doi.org/10.1371/journal.pone.0046874

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HSC70 loading control in Figures 1 and 5

Posted by h0hao001 on 30 Oct 2012 at 13:01 GMT

Due to the limited number of passages of normal melanocytes (HEMa-LP cells), adequate exosome proteins from this cell line for multiple analysis was not available. In order to save HEMa-LP exosome protein lystaes, we used the same blot to sequentially probe different antibodies in figures 1 and 5. Therefore, the same HSC70 loading control was used in these two figures.

No competing interests declared.

RE: HSC70 loading control in Figures 1 and 5

PLOS_ONE_Group replied to h0hao001 on 11 Jan 2013 at 12:52 GMT

In response the previous comment, the authors are making the raw blots for Figures 1 and 5 available via the link provided:

http://www.plosone.org/at...

Competing interests declared: PLOS ONE Staff

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Identifying mRNA, MicroRNA and Protein Profiles of Melanoma Exosomes

Identifying mRNA, MicroRNA and Protein Profiles of Melanoma Exosomes

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HSC70 loading control in Figures 1 and 5

Posted by h0hao001 on 30 Oct 2012 at 13:01 GMT

RE: HSC70 loading control in Figures 1 and 5

PLOS_ONE_Group replied to h0hao001 on 11 Jan 2013 at 12:52 GMT