Efficient and Scalable Purification of Cardiomyocytes from Human Embryonic and Induced Pluripotent Stem Cells by VCAM1 Surface Expression

Hideki Uosaki; Hiroyuki Fukushima; Ayako Takeuchi; Satoshi Matsuoka; Norio Nakatsuji; Shinya Yamanaka; Jun K. Yamashita

doi:10.1371/journal.pone.0023657

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Open Access

Peer-reviewed

Research Article

Efficient and Scalable Purification of Cardiomyocytes from Human Embryonic and Induced Pluripotent Stem Cells by VCAM1 Surface Expression

Hideki Uosaki,

Affiliations Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
⨯
Hiroyuki Fukushima,

Affiliations Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
⨯
Ayako Takeuchi,

Affiliation Department of Physiology and Biophysics, Kyoto University Graduate School of Medicine, Kyoto, Japan
⨯
Satoshi Matsuoka,

Affiliation Center for Innovation in Immunoregulative Technology and Therapeutics, Kyoto University Graduate School of Medicine, Kyoto, Japan
⨯
Norio Nakatsuji,

Affiliation Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
⨯
Shinya Yamanaka,

Affiliation Department of Reprogramming Science, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
⨯
Jun K. Yamashita

* E-mail: juny@frontier.kyoto-u.ac.jp

Affiliations Laboratory of Stem Cell Differentiation, Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan
⨯

Efficient and Scalable Purification of Cardiomyocytes from Human Embryonic and Induced Pluripotent Stem Cells by VCAM1 Surface Expression

Hideki Uosaki,
Hiroyuki Fukushima,
Ayako Takeuchi,
Satoshi Matsuoka,
Norio Nakatsuji,
Shinya Yamanaka,
Jun K. Yamashita

Published: August 18, 2011
https://doi.org/10.1371/journal.pone.0023657

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Publisher's Note: Error in the text.

Posted by PLOS_ONE_Group on 08 May 2012 at 22:23 GMT

Matrigel-coated plates at a density of 10,000 cells/cm2 in MEF-CM plus 4 ng/mL bFGF for 2–3 days before induction.
http://plosone.org/article/info:doi/10.1371/journal.pone.0023657#article1.body1.sec2.sec1.p2

There was an error in the second sentence of the second paragraph in Methods. The correct text is: ...Matrigel-coated plates at a density of 100,000 cells/cm² in MEF-CM plus 4 ng/mL bFGF for 2–3 days before induction.

No competing interests declared.

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Efficient and Scalable Purification of Cardiomyocytes from Human Embryonic and Induced Pluripotent Stem Cells by VCAM1 Surface Expression

Efficient and Scalable Purification of Cardiomyocytes from Human Embryonic and Induced Pluripotent Stem Cells by VCAM1 Surface Expression

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Publisher's Note: Error in the text.

Posted by PLOS_ONE_Group on 08 May 2012 at 22:23 GMT