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Research Article

Optimisation of Bioluminescent Reporters for Use with Mycobacteria

  • Nuria Andreu,

    Affiliation: Department of Medicine, Imperial College London, London, United Kingdom

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  • Andrea Zelmer,

    Affiliation: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

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  • Taryn Fletcher,

    Affiliation: Department of Medicine, Imperial College London, London, United Kingdom

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  • Paul T. Elkington,

    Affiliation: Department of Medicine, Imperial College London, London, United Kingdom

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  • Theresa H. Ward,

    Affiliation: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

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  • Jorge Ripoll,

    Affiliation: Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece

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  • Tanya Parish,

    Affiliations: Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom, Infectious Diseases Research Institute, Seattle, Washington, United States of America

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  • Gregory J. Bancroft,

    Affiliation: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

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  • Ulrich Schaible,

    Affiliations: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom, Department of Molecular Infection Research, Research Center Borstel, Borstel, Germany

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  • Brian D. Robertson,

    Affiliation: Department of Medicine, Imperial College London, London, United Kingdom

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  • Siouxsie Wiles mail

    s.wiles@auckland.ac.nz

    Affiliations: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom, Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand

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  • Published: May 24, 2010
  • DOI: 10.1371/journal.pone.0010777

Reader Comments (1)

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Secretion in mycobacteria

Posted by rketteler on 28 May 2010 at 11:57 GMT

A comprehensive study on different luciferases in mycobacterium tuberculosis. It might be useful to include GLUC variants with stable kinetics (Maguire et al. Anal Chem 2009; Welsh et al. BBRC 2009).
Secretion of GLUC in the absence of a signal peptide has been reported previously and seems to require association with the Golgi apparatus in mammalian cells (Ketteler et al. Genome Biol. 2008). It is highly interesting to find secretion without signal peptide in mycobacteria as well.

No competing interests declared.