Optimisation of Bioluminescent Reporters for Use with Mycobacteria

Nuria Andreu; Andrea Zelmer; Taryn Fletcher; Paul T. Elkington; Theresa H. Ward; Jorge Ripoll; Tanya Parish; Gregory J. Bancroft; Ulrich Schaible; Brian D. Robertson; Siouxsie Wiles

doi:10.1371/journal.pone.0010777

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Open Access

Peer-reviewed

Research Article

Optimisation of Bioluminescent Reporters for Use with Mycobacteria

Nuria Andreu,

Affiliation Department of Medicine, Imperial College London, London, United Kingdom
⨯
Andrea Zelmer,

Affiliation Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
⨯
Taryn Fletcher,

Affiliation Department of Medicine, Imperial College London, London, United Kingdom
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Paul T. Elkington,

Affiliation Department of Medicine, Imperial College London, London, United Kingdom
⨯
Theresa H. Ward,

Affiliation Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
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Jorge Ripoll,

Affiliation Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece
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Tanya Parish,

Affiliations Barts and the London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom, Infectious Diseases Research Institute, Seattle, Washington, United States of America
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Gregory J. Bancroft,

Affiliation Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
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Ulrich Schaible,

Affiliations Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom, Department of Molecular Infection Research, Research Center Borstel, Borstel, Germany
⨯
Brian D. Robertson,

Affiliation Department of Medicine, Imperial College London, London, United Kingdom
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Siouxsie Wiles

* E-mail: s.wiles@auckland.ac.nz

Affiliations Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom, Department of Molecular Medicine and Pathology, University of Auckland, Auckland, New Zealand
⨯

Optimisation of Bioluminescent Reporters for Use with Mycobacteria

Nuria Andreu,
Andrea Zelmer,
Taryn Fletcher,
Paul T. Elkington,
Theresa H. Ward,
Jorge Ripoll,
Tanya Parish,
Gregory J. Bancroft,
Ulrich Schaible,
Brian D. Robertson

Published: May 24, 2010
https://doi.org/10.1371/journal.pone.0010777

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Secretion in mycobacteria

Posted by rketteler on 28 May 2010 at 11:57 GMT

A comprehensive study on different luciferases in mycobacterium tuberculosis. It might be useful to include GLUC variants with stable kinetics (Maguire et al. Anal Chem 2009; Welsh et al. BBRC 2009).
Secretion of GLUC in the absence of a signal peptide has been reported previously and seems to require association with the Golgi apparatus in mammalian cells (Ketteler et al. Genome Biol. 2008). It is highly interesting to find secretion without signal peptide in mycobacteria as well.

No competing interests declared.

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Optimisation of Bioluminescent Reporters for Use with Mycobacteria

Optimisation of Bioluminescent Reporters for Use with Mycobacteria

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Secretion in mycobacteria

Posted by rketteler on 28 May 2010 at 11:57 GMT