Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag

Aimee Shen; Patrick J. Lupardus; Montse Morell; Elizabeth L. Ponder; A. Masoud Sadaghiani; K. Christopher Garcia; Matthew Bogyo

doi:10.1371/journal.pone.0008119

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Peer-reviewed

Research Article

Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag

Aimee Shen,

Affiliation Department of Pathology, Stanford School of Medicine, Stanford, California, United States of America
⨯
Patrick J. Lupardus,

Affiliation Department of Molecular and Cellular Physiology, Stanford School of Medicine, Stanford, California, United States of America
⨯
Montse Morell,

Affiliation Department of Pathology, Stanford School of Medicine, Stanford, California, United States of America
⨯
Elizabeth L. Ponder,

Affiliation Department of Pathology, Stanford School of Medicine, Stanford, California, United States of America
⨯
A. Masoud Sadaghiani,

Affiliation Department of Systems and Chemical Biology, Stanford School of Medicine, Stanford, California, United States of America
⨯
K. Christopher Garcia,

Affiliations Department of Molecular and Cellular Physiology, Stanford School of Medicine, Stanford, California, United States of America, Howard Hughes Institute, Stanford School of Medicine, Stanford, California, United States of America
⨯
Matthew Bogyo

* E-mail: mbogyo@stanford.edu

Affiliation Department of Pathology, Stanford School of Medicine, Stanford, California, United States of America
⨯

Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag

Aimee Shen,
Patrick J. Lupardus,
Montse Morell,
Elizabeth L. Ponder,
A. Masoud Sadaghiani,
K. Christopher Garcia,
Matthew Bogyo

Published: December 2, 2009
https://doi.org/10.1371/journal.pone.0008119

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Posted by eknappen on 28 Mar 2011 at 20:21 GMT

We have used this system to successfully express and purify heme peptides. Cleavage and purification are straight forward – this is a very efficient method!

No competing interests declared.

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Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag

Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag

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Posted by eknappen on 28 Mar 2011 at 20:21 GMT