Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

Kimberly A. Kelly; Jonathan Carson; Jason R. McCarthy; Ralph Weissleder

doi:10.1371/journal.pone.0000665

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Open Access

Peer-reviewed

Research Article

Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

Kimberly A. Kelly ,

Contributed equally to this work with: Kimberly A. Kelly, Jonathan Carson

Affiliation Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Jonathan Carson ,

Contributed equally to this work with: Kimberly A. Kelly, Jonathan Carson

Affiliation Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Jason R. McCarthy,

Affiliation Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Ralph Weissleder

*To whom correspondence should be addressed. E-mail: weissleder@helix.mgh.harvard.edu

Affiliation Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
⨯

Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

Kimberly A. Kelly,
Jonathan Carson,
Jason R. McCarthy,
Ralph Weissleder

Published: July 25, 2007
https://doi.org/10.1371/journal.pone.0000665

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Phage display for binding fluorescent dyes

Posted by mdi1000 on 01 Aug 2007 at 15:42 GMT

The authors describe a phage display exercise to obtain peptides that recognise a (benz)indolium fluorochrome. Molecular modeling is used to explore potential modes of binding of the selected constructs. Various in vitro and in vivo tests confirm that both phage clones and soluble peptide monomer bind the dye specifically.

The experiment appears to have been extensively controlled with various complementary methods. Most of the data appear appear to be of good quality.

The secondary candidate selected in the phage display appears to be a poly-His sequence. This would be interesting to follow-up, because His tags might be used to make fluorescent labels for imaging.

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Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

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Phage display for binding fluorescent dyes

Posted by mdi1000 on 01 Aug 2007 at 15:42 GMT