Accumulative Difference Image Protocol for Particle Tracking in Fluorescence Microscopy Tested in Mouse Lymphonodes

Carlo E. Villa; Michele Caccia; Laura Sironi; Laura D'Alfonso; Maddalena Collini; Ilaria Rivolta; Giuseppe Miserocchi; Tatiana Gorletta; Ivan Zanoni; Francesca Granucci; Giuseppe Chirico

doi:10.1371/journal.pone.0012216

Loading metrics

Open Access

Peer-reviewed

Research Article

Accumulative Difference Image Protocol for Particle Tracking in Fluorescence Microscopy Tested in Mouse Lymphonodes

Carlo E. Villa,

Affiliation Dipartimento di Fisica, Università degli Studi di Milano Bicocca, Milano, Italy
⨯
Michele Caccia,

Affiliation Dipartimento di Fisica, Università degli Studi di Milano Bicocca, Milano, Italy
⨯
Laura Sironi,

Affiliation Dipartimento di Fisica, Università degli Studi di Milano Bicocca, Milano, Italy
⨯
Laura D'Alfonso,

Affiliation Dipartimento di Fisica, Università degli Studi di Milano Bicocca, Milano, Italy
⨯
Maddalena Collini,

Affiliation Dipartimento di Fisica, Università degli Studi di Milano Bicocca, Milano, Italy
⨯
Ilaria Rivolta,

Affiliation Dipartimento di Medicina Sperimentale, Università degli Studi di Milano Bicocca, Monza, Italy
⨯
Giuseppe Miserocchi,

Affiliation Dipartimento di Medicina Sperimentale, Università degli Studi di Milano Bicocca, Monza, Italy
⨯
Tatiana Gorletta,

Affiliation Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano Bicocca, Milano, Italy
⨯
Ivan Zanoni,

Affiliation Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano Bicocca, Milano, Italy
⨯
Francesca Granucci,

Affiliation Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano Bicocca, Milano, Italy
⨯
Giuseppe Chirico

* E-mail: giuseppe.chirico@mib.infn.it

Affiliation Dipartimento di Fisica, Università degli Studi di Milano Bicocca, Milano, Italy
⨯

Accumulative Difference Image Protocol for Particle Tracking in Fluorescence Microscopy Tested in Mouse Lymphonodes

Carlo E. Villa,
Michele Caccia,
Laura Sironi,
Laura D'Alfonso,
Maddalena Collini,
Ilaria Rivolta,
Giuseppe Miserocchi,
Tatiana Gorletta,
Ivan Zanoni,
Francesca Granucci

Published: August 17, 2010
https://doi.org/10.1371/journal.pone.0012216

Reader Comments

Post a new comment on this article

Kudos to authors for open data and open code

Posted by skoch3 on 18 Sep 2010 at 21:24 GMT

The authors provide all the source code, with excellent documentation. They also provide all the raw and processed data for evaluation. I commend them for their contributions, and I hope that it helps in others building upon their image tracking work.

Competing interests declared: I served as Academic Editor for this submission.

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Lymphocytes
Is the Subject Area "Lymphocytes" applicable to this article?

Thanks for your feedback.
Algorithms
Is the Subject Area "Algorithms" applicable to this article?

Thanks for your feedback.
Target detection
Is the Subject Area "Target detection" applicable to this article?

Thanks for your feedback.
In vivo imaging
Is the Subject Area "In vivo imaging" applicable to this article?

Thanks for your feedback.
Fluorescence imaging
Is the Subject Area "Fluorescence imaging" applicable to this article?

Thanks for your feedback.
Nanoparticles
Is the Subject Area "Nanoparticles" applicable to this article?

Thanks for your feedback.
Computer software
Is the Subject Area "Computer software" applicable to this article?

Thanks for your feedback.
Imaging techniques
Is the Subject Area "Imaging techniques" applicable to this article?

Thanks for your feedback.

Accumulative Difference Image Protocol for Particle Tracking in Fluorescence Microscopy Tested in Mouse Lymphonodes

Accumulative Difference Image Protocol for Particle Tracking in Fluorescence Microscopy Tested in Mouse Lymphonodes

Reader Comments

Post Your Discussion Comment

Why should this posting be reviewed?

Thank You!

Kudos to authors for open data and open code

Posted by skoch3 on 18 Sep 2010 at 21:24 GMT