Advertisement
Research Article

The Neglected Intrinsic Resistome of Bacterial Pathogens

  • Alicia Fajardo,

    Affiliation: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Cantoblanco, Madrid, Spain

    X
  • Nadia Martínez-Martín,

    Affiliation: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Cantoblanco, Madrid, Spain

    X
  • María Mercadillo,

    Affiliation: Unidad Asociada al Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC) “Resistencia a los antibióticos y virulencia bacteriana”, Hospital Ramón y Cajal, Madrid, Spain

    X
  • Juan C. Galán,

    Affiliations: Unidad Asociada al Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC) “Resistencia a los antibióticos y virulencia bacteriana”, Hospital Ramón y Cajal, Madrid, Spain, CIBER Epidemiología y Salud Pública (CIBERESP), Hospital Ramón y Cajal, Madrid, Spain, Departamento de Microbiología, Hospital Ramón y Cajal, Madrid, Spain

    X
  • Bart Ghysels,

    Affiliation: Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium

    X
  • Sandra Matthijs,

    Affiliation: Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium

    X
  • Pierre Cornelis,

    Affiliation: Laboratory of Microbial Interactions, Department of Molecular and Cellular Interactions, Flanders Interuniversity Institute for Biotechnology, Vrije Universiteit Brussel, Brussels, Belgium

    X
  • Lutz Wiehlmann,

    Affiliation: Klinische Forschergruppe, Medizinische Hochschule Hannover, Hannover, Germany

    X
  • Burkhard Tümmler,

    Affiliation: Klinische Forschergruppe, Medizinische Hochschule Hannover, Hannover, Germany

    X
  • Fernando Baquero,

    Affiliations: Unidad Asociada al Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC) “Resistencia a los antibióticos y virulencia bacteriana”, Hospital Ramón y Cajal, Madrid, Spain, CIBER Epidemiología y Salud Pública (CIBERESP), Hospital Ramón y Cajal, Madrid, Spain, Departamento de Microbiología, Hospital Ramón y Cajal, Madrid, Spain

    X
  • José L. Martínez mail

    To whom correspondence should be addressed. E-mail: jlmtnez@cnb.uam.es

    Affiliations: Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Cantoblanco, Madrid, Spain, Unidad Asociada al Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC) “Resistencia a los antibióticos y virulencia bacteriana”, Hospital Ramón y Cajal, Madrid, Spain, CIBER Epidemiología y Salud Pública (CIBERESP), Hospital Ramón y Cajal, Madrid, Spain

    X
  • Published: February 20, 2008
  • DOI: 10.1371/journal.pone.0001619

Reader Comments (4)

Post a new comment on this article

An interesting set of experiments with both strengths and weaknesses.

Posted by Adam on 10 Mar 2008 at 00:26 GMT


Comment originally posted by me at gramstain.blogspot.com:

A recent article in PLoS ONE takes a broad look at antibiotic resistance in Pseudomonas aeruginosa, an environmental organism and a cause of opportunistic infections. Pseudomonas infections are particularly difficult to treat, as it is frequently resistant to numerous classes of antibiotics. Children who are predisposed to chronic colonization or infection with Pseudomonas, such as those with cystic fibrosis, may eventually harbor bacteria that are resistant to all available antibiotics. Fajardo et al. (citation below) screened two transposon libraries of P. aeruginosa looking for genes that either increased or decreased susceptibility to a panel of antibiotics. Their results are of interest, as many of their hits (genes associated with a change in susceptibility) were in classes of genes not previously linked to resistance. A weakness of the study is that they do not go on to make defined mutations in these genes or to complement the phenotype by expressing the mutated gene on a plasmid, but it is an interesting screen that has the potential to provide targets for future antimicrobial development.