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Research Article

Iridovirus and Microsporidian Linked to Honey Bee Colony Decline

  • Jerry J. Bromenshenk mail,

    beeresearch@aol.com

    Affiliations: Division of Biological Sciences, The University of Montana, Missoula, Montana, United States of America, Bee Alert Technology, Inc., Missoula, Montana, United States of America

    X
  • Colin B. Henderson,

    Affiliations: College of Technology, The University of Montana, Missoula, Montana, United States of America, Bee Alert Technology, Inc., Missoula, Montana, United States of America

    X
  • Charles H. Wick,

    Affiliation: US Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Edgewood Area, Maryland, United States of America

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  • Michael F. Stanford,

    Affiliation: US Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Edgewood Area, Maryland, United States of America

    X
  • Alan W. Zulich,

    Affiliation: US Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Edgewood Area, Maryland, United States of America

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  • Rabih E. Jabbour,

    Affiliation: Science Applications International Corporation, Abingdon, Maryland, United States of America

    X
  • Samir V. Deshpande,

    Affiliations: Science Technology Corporation, Edgewood, Maryland, United States of America, Department of Computer and Information Sciences, Towson University, Towson, Maryland, United States of America

    X
  • Patrick E. McCubbin,

    Affiliation: OptiMetrics, Inc., Abingdon, Maryland, United States of America

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  • Robert A. Seccomb,

    Affiliation: Bee Alert Technology, Inc., Missoula, Montana, United States of America

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  • Phillip M. Welch,

    Affiliation: Bee Alert Technology, Inc., Missoula, Montana, United States of America

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  • Trevor Williams,

    Affiliation: Instituto de Ecologia AC, Xalapa, Veracruz, Mexico

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  • David R. Firth,

    Affiliation: Department of Information Systems and Technology, The University of Montana, Missoula, Montana, United States of America

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  • Evan Skowronski,

    Affiliation: US Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Edgewood Area, Maryland, United States of America

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  • Margaret M. Lehmann,

    Affiliation: Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America

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  • Shan L. Bilimoria,

    Affiliations: Department of Biological Sciences, Texas Tech University, Lubbock, Texas, United States of America, Center for Biotechnology and Genomics, Texas Tech University, Lubbock, Texas, United States of America

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  • Joanna Gress,

    Affiliation: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, Montana, United States of America

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  • Kevin W. Wanner,

    Affiliation: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, Montana, United States of America

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  • Robert A. Cramer Jr

    Affiliation: Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America

    X
  • Published: October 06, 2010
  • DOI: 10.1371/journal.pone.0013181

Reader Comments (14)

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Publication of re-analysis of a subset of the raw data

Posted by chalkley on 14 Jun 2011 at 23:11 GMT

We have just published an article in PLoS One that is a re-analysis of the raw mass spectrometry data from three of the fractions in this Bromenshenk et al study:
http://www.plosone.org/ar...
We gained access to three of the raw data files (Xcalibur .raw files) from Dr Wick, one of the authors on the original study. By searching the data using standard search parameters against the whole NCBInr database, we found no evidence that peptides from Iridovirus and Nosema were present in these fractions, but were able to re-interpret a significant percentage of the spectra assigned to viral peptides as being peptides derived from bee proteins.
We have made the raw data from these fractions available on Tranche if other people wish to independently re-assess the data.

No competing interests declared.