The Polycomb Protein and E3 Ubiquitin Ligase Ring1B Harbors an IRES in its Highly Conserved 5′ UTR

Erwin Boutsma; Sonja Noback; Maarten van Lohuizen

doi:10.1371/journal.pone.0002322

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Peer-reviewed

Research Article

The Polycomb Protein and E3 Ubiquitin Ligase Ring1B Harbors an IRES in its Highly Conserved 5′ UTR

Erwin Boutsma,

Affiliation Division of Molecular Genetics, Netherlands Cancer Institute, Amsterdam, The Netherlands
⨯
Sonja Noback,

Affiliation Division of Molecular Genetics, Netherlands Cancer Institute, Amsterdam, The Netherlands
⨯
Maarten van Lohuizen

* E-mail: m.v.lohuizen@nki.nl

Affiliation Division of Molecular Genetics, Netherlands Cancer Institute, Amsterdam, The Netherlands
⨯

The Polycomb Protein and E3 Ubiquitin Ligase Ring1B Harbors an IRES in its Highly Conserved 5′ UTR

Erwin Boutsma,
Sonja Noback,
Maarten van Lohuizen

Published: June 4, 2008
https://doi.org/10.1371/journal.pone.0002322

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IRES or not?

Posted by MartinH on 05 Jun 2008 at 18:06 GMT

An interesting observation if indeed an IRES is present in the 5' UTR. However, no data is presented on the integrity of bicistronic mRNA so the possibility exists that the observed IRES activity is in fact just splicing of the construct. This would also apply to the 5'UTR-Ring1B construct where splicing would remove 5'UTR and make the mRNA more efficiently translated due to processing, and probably deposition of a EJC proteins.

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The Polycomb Protein and E3 Ubiquitin Ligase Ring1B Harbors an IRES in its Highly Conserved 5′ UTR

The Polycomb Protein and E3 Ubiquitin Ligase Ring1B Harbors an IRES in its Highly Conserved 5′ UTR

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IRES or not?

Posted by MartinH on 05 Jun 2008 at 18:06 GMT