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Research Article

Drug-Resistant Genotypes and Multi-Clonality in Plasmodium falciparum Analysed by Direct Genome Sequencing from Peripheral Blood of Malaria Patients

  • Timothy Robinson equal contributor,

    equal contributor Contributed equally to this work with: Timothy Robinson, Susana G. Campino

    Affiliation: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom

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  • Susana G. Campino equal contributor,

    equal contributor Contributed equally to this work with: Timothy Robinson, Susana G. Campino

    Affiliation: Wellcome Trust Sanger Institute, Hinxton, United Kingdom

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  • Sarah Auburn,

    Affiliations: Wellcome Trust Sanger Institute, Hinxton, United Kingdom, Global Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Australia

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  • Samuel A. Assefa,

    Affiliation: Wellcome Trust Sanger Institute, Hinxton, United Kingdom

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  • Spencer D. Polley,

    Affiliation: Department of Clinical Parasitology, Hospital for Tropical Diseases, London, United Kingdom

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  • Magnus Manske,

    Affiliation: Wellcome Trust Sanger Institute, Hinxton, United Kingdom

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  • Bronwyn MacInnis,

    Affiliations: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom, Wellcome Trust Sanger Institute, Hinxton, United Kingdom

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  • Kirk A. Rockett,

    Affiliations: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom, Wellcome Trust Sanger Institute, Hinxton, United Kingdom

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  • Gareth L. Maslen,

    Affiliation: Wellcome Trust Sanger Institute, Hinxton, United Kingdom

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  • Mandy Sanders,

    Affiliation: Wellcome Trust Sanger Institute, Hinxton, United Kingdom

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  • Michael A. Quail,

    Affiliation: Wellcome Trust Sanger Institute, Hinxton, United Kingdom

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  • Peter L. Chiodini,

    Affiliations: Department of Clinical Parasitology, Hospital for Tropical Diseases, London, United Kingdom, Faculties of Infectious and Tropical Diseases and Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, London, United Kingdom

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  • Dominic P. Kwiatkowski,

    Affiliations: Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom, Wellcome Trust Sanger Institute, Hinxton, United Kingdom

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  • Taane G. Clark,

    Affiliation: Faculties of Infectious and Tropical Diseases and Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, London, United Kingdom

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  • Colin J. Sutherland mail

    colin.sutherland@lshtm.ac.uk

    Affiliations: Department of Clinical Parasitology, Hospital for Tropical Diseases, London, United Kingdom, Faculties of Infectious and Tropical Diseases and Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, London, United Kingdom

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  • Published: August 11, 2011
  • DOI: 10.1371/journal.pone.0023204

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A brief note from the authors

Posted by Colins on 24 Aug 2011 at 08:56 GMT

Dear PLoS ONE Readers,

The intention of this paper is to provide an accessible "proof-of-principle" introduction to massively parallel sequencing (MPS) studies of the malaria parasite "italic" Plasmodium falciparum "end italic", using a small number of well-characterised patient isolates. We therefore focussed on a few questions familiar to malariologists:

- How many clones are present in this patient?

- Which drug resistant genotypes are present?

- Are there gene deletions or amplifications?

- Can we extract comprehensive genetic data from the mitochondrial genome using MPS?

We would welcome comments and suggestions from readers to inform our approach to future papers, particularly with regard to accessibility of the methodology used.

Competing interests declared: Corresponding author of the article.