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How to dissolve PVA

Posted by pwburridge on 04 Aug 2011 at 18:07 GMT

A common question is how to dissolve the PVA used for the hEB formation step.

To make 500 mL of RPMI1640 with 4mg/mL PVA:

Weigh 2g of PVA. Under the tissue culture hood pour the PVA in to a new, cold 500 mL bottle of RPMI, mix by inversion and place at 4*C for 72 h.

The RPMI with PVA can be kept at 4*C until required, when the media should be made and sterile filtered as normal.

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