pp32 (ANP32A) Expression Inhibits Pancreatic Cancer Cell Growth and Induces Gemcitabine Resistance by Disrupting HuR Binding to mRNAs

Timothy K. Williams; Christina L. Costantino; Nikolai A. Bildzukewicz; Nathan G. Richards; David W. Rittenhouse; Lisa Einstein; Joseph A. Cozzitorto; Judith C. Keen; Abhijit Dasgupta; Myriam Gorospe; Gregory E. Gonye; Charles J. Yeo; Agnieszka K. Witkiewicz; Jonathan R. Brody

doi:10.1371/journal.pone.0015455

Loading metrics

Open Access

Peer-reviewed

Research Article

pp32 (ANP32A) Expression Inhibits Pancreatic Cancer Cell Growth and Induces Gemcitabine Resistance by Disrupting HuR Binding to mRNAs

Timothy K. Williams ,

Contributed equally to this work with: Timothy K. Williams, Christina L. Costantino

Affiliation Department of Surgery, Jefferson Pancreas, Biliary and Related Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Christina L. Costantino ,

Contributed equally to this work with: Timothy K. Williams, Christina L. Costantino

Affiliation Department of Surgery, Jefferson Pancreas, Biliary and Related Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Nikolai A. Bildzukewicz,

Affiliation Department of Surgery, Jefferson Pancreas, Biliary and Related Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Nathan G. Richards,

Affiliation Department of Surgery, Jefferson Pancreas, Biliary and Related Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
David W. Rittenhouse,

Affiliation Department of Surgery, Jefferson Pancreas, Biliary and Related Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Lisa Einstein,

Affiliation Department of Surgery, Jefferson Pancreas, Biliary and Related Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Joseph A. Cozzitorto,

Affiliation Department of Surgery, Jefferson Pancreas, Biliary and Related Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Judith C. Keen,

Affiliation Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Camden, New Jersey, United States of America
⨯
Abhijit Dasgupta,

Affiliations Department of Pathology, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Myriam Gorospe,

Affiliation Laboratory of Cellular and Molecular Biology, National Institute on Aging-Intramural Research Program, National Institutes of Health, Baltimore, Maryland, United States of America
⨯
Gregory E. Gonye,

Affiliation Department of Pathology, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Charles J. Yeo,

Affiliations Department of Surgery, Jefferson Pancreas, Biliary and Related Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Agnieszka K. Witkiewicz,

Affiliations Department of Surgery, Jefferson Pancreas, Biliary and Related Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America, Department of Pathology, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯
Jonathan R. Brody

* E-mail: Jonathan.Brody@jefferson.edu

Affiliations Department of Surgery, Jefferson Pancreas, Biliary and Related Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America, Department of Pathology, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of America
⨯

pp32 (ANP32A) Expression Inhibits Pancreatic Cancer Cell Growth and Induces Gemcitabine Resistance by Disrupting HuR Binding to mRNAs

Timothy K. Williams,
Christina L. Costantino,
Nikolai A. Bildzukewicz,
Nathan G. Richards,
David W. Rittenhouse,
Lisa Einstein,
Joseph A. Cozzitorto,
Judith C. Keen,
Abhijit Dasgupta,
Myriam Gorospe

Published: November 29, 2010
https://doi.org/10.1371/journal.pone.0015455

Reader Comments

Post a new comment on this article

Correction and thank you: pp32 is a phosphoprotein

Posted by Jbrody on 25 Apr 2011 at 23:22 GMT

We thank Dr. Patrick Reilly for his thorough reading of the manuscript and abstract. Indeed, a correction should be noted that was missed during the final edits. PP for the pp32 portion of the name is a "phosphoprotein"and not "protein phosphatase." We appreciate this note, as the last author was part of the initial cloning of the gene family and we appreciate keeping the full integrity of the function of the gene intact. Thank you again for giving attention to this matter. We note the only correlation is that pp32 has been cited as potent inhibitor of PP2A (PHAP-I).

No competing interests declared.

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Pancreatic cancer
Is the Subject Area "Pancreatic cancer" applicable to this article?

Thanks for your feedback.
Transfection
Is the Subject Area "Transfection" applicable to this article?

Thanks for your feedback.
Malignant tumors
Is the Subject Area "Malignant tumors" applicable to this article?

Thanks for your feedback.
Cancer treatment
Is the Subject Area "Cancer treatment" applicable to this article?

Thanks for your feedback.
Small interfering RNA
Is the Subject Area "Small interfering RNA" applicable to this article?

Thanks for your feedback.
Cancers and neoplasms
Is the Subject Area "Cancers and neoplasms" applicable to this article?

Thanks for your feedback.
Immunoprecipitation
Is the Subject Area "Immunoprecipitation" applicable to this article?

Thanks for your feedback.
Gene expression
Is the Subject Area "Gene expression" applicable to this article?

Thanks for your feedback.

pp32 (ANP32A) Expression Inhibits Pancreatic Cancer Cell Growth and Induces Gemcitabine Resistance by Disrupting HuR Binding to mRNAs

pp32 (ANP32A) Expression Inhibits Pancreatic Cancer Cell Growth and Induces Gemcitabine Resistance by Disrupting HuR Binding to mRNAs

Reader Comments

Post Your Discussion Comment

Why should this posting be reviewed?

Thank You!

Correction and thank you: pp32 is a phosphoprotein

Posted by Jbrody on 25 Apr 2011 at 23:22 GMT