Referee 1's review (Catherine E. Krull):
In this manuscript, the authors describe using electroporation to label cells in the olfactory system of the postnatal rodent. Overall, the approach is well-described and labels multiple cell types. Furthermore, this approach should be very useful in delivering shRNAs and controlling the timing of protein disruption. I have a couple comments and these are listed below:
1. Did the authors check cell death following electroporation, using TUNEL for example?
2. Is the concentration of their plasmid really 1-5 ug/ml? In chick, it is typical to use highly concentrated DNA (1-5 ug/ul).
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N.B. These are the comments made by the referee when reviewing an earlier version of this paper. Prior to publication the manuscript has been revised in light of these comments and to address other editorial requirements.
