Mapping Accuracy of Short Reads from Massively Parallel Sequencing and the Implications for Quantitative Expression Profiling

Open Access

Peer-reviewed

Research Article

Nicola Palmieri,

Affiliation Institut für Populationsgenetik, Veterinärmedizinische Universität Wien, Vienna, Austria
⨯
Christian Schlötterer

* E-mail: christian.schloetterer@vu-wien.ac.at

Affiliation Institut für Populationsgenetik, Veterinärmedizinische Universität Wien, Vienna, Austria
⨯

Reader Comments

.
http://plosone.org/article/info:doi/10.1371/journal.pone.0006323#article1.back1.ack1.p1

We are thankful to Ben Langmead and Ido Tamir for pointing out that we overlooked the option in MAQ and Bowtie to ignore ambiguously mapped reads. We apologize for this negligence.

No competing interests declared.

Invertebrate genomics
Is the Subject Area "Invertebrate genomics" applicable to this article?

Thanks for your feedback.
Gene expression
Is the Subject Area "Gene expression" applicable to this article?

Thanks for your feedback.
Plant genomics
Is the Subject Area "Plant genomics" applicable to this article?

Thanks for your feedback.
Computer software
Is the Subject Area "Computer software" applicable to this article?

Thanks for your feedback.
Drosophila melanogaster
Is the Subject Area "Drosophila melanogaster" applicable to this article?

Thanks for your feedback.
Gene mapping
Is the Subject Area "Gene mapping" applicable to this article?

Thanks for your feedback.
Human genomics
Is the Subject Area "Human genomics" applicable to this article?

Thanks for your feedback.
Insertion mutation
Is the Subject Area "Insertion mutation" applicable to this article?

Thanks for your feedback.