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expression in the locus ceruleus

Posted by gautam on 08 Feb 2010 at 15:21 GMT

I very much enjoyed your PLOS one paper on GSK-3beta.

I wonder if there was any GSK-3beta labeling in the locus ceruleus. I was not able to tell from the manuscript whether you had examined that region (rostral pons).

Thanks Very Much for Your Time,

No competing interests declared.

RE: expression in the locus ceruleus

gautam replied to gautam on 08 Feb 2010 at 15:23 GMT

Thank you for your interest in our paper on GSK3b expression in the mouse brain. I am writing you concerning your question on the expression of GSK3b in the locus coeruleus. You are certainly right that is not described in detail in the paper (there is an abundance of nuclei that express GSK3b on that area and our paper was already considerably detailed and lengthy, so it was beyond the scope of the work to include more detail)
However, since we have the slides to check, we went yesterday and reviewed them to answer your question and the answer to your question is as follows:

* At the light microscope, GSK3b labeling is observed as moderate to intense labeling in the cytoplasm and in the initial segment of some processes of neurons of the Nucleus Coeruleus. The nuclei of these labeled neurons is devoid of labeling at the light microscope. We could not distinguish any glia staining in this area at the light microscope with GSK3b antibodies (the constitutively active GSK3b)


* In the case of pSer9GSK3b labeling: the labeling at the light microscope shows clearly the presence of abundant microglia cells that appear labeled in the cytoplasm and processes in a similar manner to microglia cells shown for other regions of the brain in our paper. No neuronal staining is observed for pser9GSK3b in the Locus Coeruleus at the ligth microscope.

On behalf of all the authors of the paper I wish to thank you for you interest in our paper and please do not hesitate to contact us if you have any more questions or comments concerning our work.


Best Regards,

--
Emma Perez-Costas, PhD
Assistant Professor
Department of Psychiatry and Behavioral Neurobiology
University of Alabama at Birmingham
epcostas@uab.edu

No competing interests declared.

RE: RE: expression in the locus ceruleus

gautam replied to gautam on 08 Feb 2010 at 15:26 GMT

This inquiry was submitted to us by email. We felt that it should be posted in the comments section as the question and answer may be relevant to other researchers.

No competing interests declared.