Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler; Ramona Gruetzner; Romy Kandzia; Sylvestre Marillonnet

doi:10.1371/journal.pone.0005553

Loading metrics

Open Access

Peer-reviewed

Research Article

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler ,

Contributed equally to this work with: Carola Engler, Ramona Gruetzner

Affiliation Icon Genetics GmbH, Biozentrum Halle, Halle, Germany
⨯
Ramona Gruetzner ,

Contributed equally to this work with: Carola Engler, Ramona Gruetzner

Affiliation Icon Genetics GmbH, Biozentrum Halle, Halle, Germany
⨯
Romy Kandzia,

Affiliation Icon Genetics GmbH, Biozentrum Halle, Halle, Germany
⨯
Sylvestre Marillonnet

* E-mail: marillonnet@icongenetics.de

Affiliation Icon Genetics GmbH, Biozentrum Halle, Halle, Germany
⨯

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler,
Ramona Gruetzner,
Romy Kandzia,
Sylvestre Marillonnet

Published: May 14, 2009
https://doi.org/10.1371/journal.pone.0005553

Reader Comments

Post a new comment on this article

Potential use for gene synthesis

Posted by peccoud on 27 May 2009 at 12:56 GMT

This cloning technique could be used to assemble building blocks in gene synthesis projects. We have recently reviewed this field available at http://www.cell.com/trend...(08)00285-0

No competing interests declared.

RE: Potential use for gene synthesis

peccoud replied to peccoud on 27 May 2009 at 13:06 GMT

The link is broken. http://tinyurl.com/genesy... should work better.

No competing interests declared.

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Cloning
Is the Subject Area "Cloning" applicable to this article?

Thanks for your feedback.
Plasmid construction
Is the Subject Area "Plasmid construction" applicable to this article?

Thanks for your feedback.
DNA cloning
Is the Subject Area "DNA cloning" applicable to this article?

Thanks for your feedback.
Ligases
Is the Subject Area "Ligases" applicable to this article?

Thanks for your feedback.
Polymerase chain reaction
Is the Subject Area "Polymerase chain reaction" applicable to this article?

Thanks for your feedback.
DNA fragment ligation
Is the Subject Area "DNA fragment ligation" applicable to this article?

Thanks for your feedback.
Nucleotide sequencing
Is the Subject Area "Nucleotide sequencing" applicable to this article?

Thanks for your feedback.
Vector cloning
Is the Subject Area "Vector cloning" applicable to this article?

Thanks for your feedback.

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Reader Comments

Post Your Discussion Comment

Why should this posting be reviewed?

Thank You!

Potential use for gene synthesis

Posted by peccoud on 27 May 2009 at 12:56 GMT

RE: Potential use for gene synthesis

peccoud replied to peccoud on 27 May 2009 at 13:06 GMT