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Research Article

Iridovirus and Microsporidian Linked to Honey Bee Colony Decline

  • Jerry J. Bromenshenk mail,

    beeresearch@aol.com

    Affiliations: Division of Biological Sciences, The University of Montana, Missoula, Montana, United States of America, Bee Alert Technology, Inc., Missoula, Montana, United States of America

    X
  • Colin B. Henderson,

    Affiliations: College of Technology, The University of Montana, Missoula, Montana, United States of America, Bee Alert Technology, Inc., Missoula, Montana, United States of America

    X
  • Charles H. Wick,

    Affiliation: US Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Edgewood Area, Maryland, United States of America

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  • Michael F. Stanford,

    Affiliation: US Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Edgewood Area, Maryland, United States of America

    X
  • Alan W. Zulich,

    Affiliation: US Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Edgewood Area, Maryland, United States of America

    X
  • Rabih E. Jabbour,

    Affiliation: Science Applications International Corporation, Abingdon, Maryland, United States of America

    X
  • Samir V. Deshpande,

    Affiliations: Science Technology Corporation, Edgewood, Maryland, United States of America, Department of Computer and Information Sciences, Towson University, Towson, Maryland, United States of America

    X
  • Patrick E. McCubbin,

    Affiliation: OptiMetrics, Inc., Abingdon, Maryland, United States of America

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  • Robert A. Seccomb,

    Affiliation: Bee Alert Technology, Inc., Missoula, Montana, United States of America

    X
  • Phillip M. Welch,

    Affiliation: Bee Alert Technology, Inc., Missoula, Montana, United States of America

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  • Trevor Williams,

    Affiliation: Instituto de Ecologia AC, Xalapa, Veracruz, Mexico

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  • David R. Firth,

    Affiliation: Department of Information Systems and Technology, The University of Montana, Missoula, Montana, United States of America

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  • Evan Skowronski,

    Affiliation: US Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, Edgewood Area, Maryland, United States of America

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  • Margaret M. Lehmann,

    Affiliation: Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America

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  • Shan L. Bilimoria,

    Affiliations: Department of Biological Sciences, Texas Tech University, Lubbock, Texas, United States of America, Center for Biotechnology and Genomics, Texas Tech University, Lubbock, Texas, United States of America

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  • Joanna Gress,

    Affiliation: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, Montana, United States of America

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  • Kevin W. Wanner,

    Affiliation: Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, Montana, United States of America

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  • Robert A. Cramer Jr

    Affiliation: Department of Veterinary Molecular Biology, Montana State University, Bozeman, Montana, United States of America

    X
  • Published: October 06, 2010
  • DOI: 10.1371/journal.pone.0013181

Reader Comments (14)

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Request Technical Report

Posted by beeresearch on 18 Oct 2010 at 16:43 GMT

The US Army technical report was written and assigned a publication number before our PLoS ONE article was accepted. The final edits and clearance for publication by the Army is taking longer than anticipated.

We have discussed this with the PLoS ONE Executive Editor and offered to post the raw data from our study on PLoS ONE. The journal is looking into how best to publish this 2,000 line Excel file.

In the meantime, we have been asked by the Executive Editor to inform readers that the easiest approach would be for us to simply send the Excel file to those who request it.

We ask that you cc requests to Dr. Walter Leal, the Academic Editor, so that he can establish a master list.





No competing interests declared.

RE: Request Technical Report

beeresearch replied to beeresearch on 18 Oct 2010 at 16:50 GMT

I checked the Comment posting and realize that my e-mail address was not readily apparent.

Please submit requests for the PLoS ONE Excel file to Dr. Colin Henderson at colin.henderson@mso.umt.edu.

Please cc to me, Jerry Bromenshenk, at beeresearch@aol.com AND to
Dr. Walter Leal at wsleal@ucdavis.edu

No competing interests declared.

RE: RE: Request Technical Report

chalkley replied to beeresearch on 19 Nov 2010 at 02:50 GMT

I don’t think the Excel spreadsheet is going to satisfy people’s concerns. What people want to be able to do is assess the reliability of the results. To do this, they need to know sufficient details about how the analysis was done and have access to the data to search themselves.
The PLOS manuscript does not contain sufficient information to assess the reliability of the mass spectrometry data. The proteomics community, with support from all journals who regularly publish this type of analysis, have developed journal publication guidelines that outline minimal amounts of information required to be able to assess mass spectrometry-based proteomic results. These have been in place for five years and the most up-to-date guidelines can be found here:
http://mcponline.org/site...
These guidelines require reporting all data search parameters and they also now require submitting of raw data to a public repository. It should be straightforward to submit the raw data (or at least the mass spectrometry peak lists that were used for analysis) and the excel results spreadsheet to e.g. Tranche, then PLOS can link to the raw data in Tranche through the hash created (see final guideline in the link above). This will allow anyone free access to the data.

No competing interests declared.