Distinct Patterns of DNA Damage Response and Apoptosis Correlate with Jak/Stat and PI3Kinase Response Profiles in Human Acute Myelogenous Leukemia

David B. Rosen; Santosh Putta; Todd Covey; Ying-Wen Huang; Garry P. Nolan; Alessandra Cesano; Mark D. Minden; Wendy J. Fantl

doi:10.1371/journal.pone.0012405

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Peer-reviewed

Research Article

Distinct Patterns of DNA Damage Response and Apoptosis Correlate with Jak/Stat and PI3Kinase Response Profiles in Human Acute Myelogenous Leukemia

David B. Rosen,

Affiliation Nodality, Inc., South San Francisco, California, United States of America
⨯
Santosh Putta,

Affiliation Nodality, Inc., South San Francisco, California, United States of America
⨯
Todd Covey,

Affiliation Nodality, Inc., South San Francisco, California, United States of America
⨯
Ying-Wen Huang,

Affiliation Nodality, Inc., South San Francisco, California, United States of America
⨯
Garry P. Nolan,

Affiliation Baxter Laboratory for Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, California, United States of America
⨯
Alessandra Cesano,

Affiliation Nodality, Inc., South San Francisco, California, United States of America
⨯
Mark D. Minden,

Affiliation Princess Margaret Hospital, Toronto, Ontario, Canada
⨯
Wendy J. Fantl

* E-mail: wjfantl@stanford.edu

Affiliations Baxter Laboratory for Stem Cell Biology, Department of Microbiology and Immunology, Stanford University, Stanford, California, United States of America, Nodality, Inc., South San Francisco, California, United States of America
⨯

Distinct Patterns of DNA Damage Response and Apoptosis Correlate with Jak/Stat and PI3Kinase Response Profiles in Human Acute Myelogenous Leukemia

David B. Rosen,
Santosh Putta,
Todd Covey,
Ying-Wen Huang,
Garry P. Nolan,
Alessandra Cesano,
Mark D. Minden,
Wendy J. Fantl

Published: August 25, 2010
https://doi.org/10.1371/journal.pone.0012405

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Pan Metrics Ariston!

Posted by IOANNIS on 18 Sep 2010 at 19:17 GMT

Comment 1.
The use of MFI as a metric occurs strange to me, since
the figures clearly demonstrate distinct subpopulations (i.e. STAT3+/5+) and they also display the percentages on each plot. Wouldn't it be better to use the percentages of well characterized cell populations - after all, this is the power of cytometry in single cell analysis?

Comment 2.
The blast 1 subpopulation in figure 6 seems to be comprised by immature precursors (myelocytes and so on) rather than "real" blasts. At least morphologically CD45brightCD33+ cells are usually not blasts, although they probably belong to the malignant clone (depending on the subtype of AML and ...the leukemogenesis model that one supports).

No competing interests declared.

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Distinct Patterns of DNA Damage Response and Apoptosis Correlate with Jak/Stat and PI3Kinase Response Profiles in Human Acute Myelogenous Leukemia

Distinct Patterns of DNA Damage Response and Apoptosis Correlate with Jak/Stat and PI3Kinase Response Profiles in Human Acute Myelogenous Leukemia

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Pan Metrics Ariston!

Posted by IOANNIS on 18 Sep 2010 at 19:17 GMT