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Research Article

Anti-calmodulins and Tricyclic Adjuvants in Pain Therapy Block the TRPV1 Channel

  • Zoltán Oláh mail,

    To whom correspondence should be addressed. E-mail: zolah@brc.hu

    Affiliations: Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary, Acheuron Hungary Ltd., Szeged, Hungary, Acheuron Pharmaceuticals Inc., San Diego, California, United States of America

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  • Katalin Jósvay,

    Affiliation: Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary

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  • László Pecze,

    Affiliation: Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary

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  • Tamás Letoha,

    Affiliations: Department of Medical Chemistry, Faculty of General Medicine, University of Szeged, Szeged, Hungary, Acheuron Hungary Ltd., Szeged, Hungary

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  • Norbert Babai,

    Affiliation: Department of Experimental Zoology and Neurobiology, University of Pécs, Pécs, Hungary

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  • Dénes Budai,

    Affiliation: Department of Biology, Gyula Juh‡sz Faculty of Education, University of Szeged, Szeged, Hungary

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  • Ferenc Ötvös,

    Affiliation: Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary

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  • Sándor Szalma,

    Affiliation: Acheuron Pharmaceuticals Inc., San Diego, California, United States of America

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  • Csaba Vizler

    Affiliation: Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary

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  • Published: June 20, 2007
  • DOI: 10.1371/journal.pone.0000545

Reader Comments (2)

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Referee Comments: Referee 1

Posted by PLoS_ONE_Group on 26 Jun 2007 at 13:06 GMT

Reviewer 1's Review

“The ms. reports findings of possible general interest, which were generated by a respectable group of investigators, following up on an interesting and novel idea.

However, there are two major shortcomings that significantly subtract from enthusiasm for this investigation.

1 - there is a lack of clarity of presentation, of familiarizing the reader with the experimental ductus, of explaining the results of the authors' own experiments and, subsequently, of interpreting them in the context of existing knowledge generated by others. In the end: what is the significant advance carried by their study and why does it have general impact ? Apart from the thorough re-write, weeding out minor blemishes, this desired clarity is what is missing in the first place.

2 - the choice of embryonic DRG neurons is not clear to this reviewer. Adult trigeminal or DRG sensory neurons could have been used in dissociated cultures as a cellular model of nociceptive neuron. This has been accomplished by many authors (e.g. Hargreaves, Schaible, Simon and many others) and would have represented a more valid model than the reported embryonic sensory neuron culture.”

N.B. These are the general comments made by the reviewer when reviewing this paper in light of which the manuscript was revised. Specific points addressed during revision of the paper are not shown.