Evaluation of Microarray Preprocessing Algorithms Based on Concordance with RT-PCR in Clinical Samples

Balazs Gyorffy; Bela Molnar; Hermann Lage; Zoltan Szallasi; Aron C. Eklund

doi:10.1371/journal.pone.0005645

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Open Access

Peer-reviewed

Research Article

Evaluation of Microarray Preprocessing Algorithms Based on Concordance with RT-PCR in Clinical Samples

Balazs Gyorffy,

Affiliation Hungarian Academy of Sciences and Semmelweis University Budapest, Budapest, Hungary
⨯
Bela Molnar,

Affiliation Hungarian Academy of Sciences and Semmelweis University Budapest, Budapest, Hungary
⨯
Hermann Lage,

Affiliation Charité Universitätsmedizin Berlin, Institute of Pathology, Berlin, Germany
⨯
Zoltan Szallasi,

Affiliations Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark, Children's Hospital Boston, Boston, Massachusetts, United States of America
⨯
Aron C. Eklund

* E-mail: eklund@cbs.dtu.dk

Affiliations Center for Biological Sequence Analysis, Technical University of Denmark, Lyngby, Denmark, Children's Hospital Boston, Boston, Massachusetts, United States of America
⨯

Evaluation of Microarray Preprocessing Algorithms Based on Concordance with RT-PCR in Clinical Samples

Balazs Gyorffy,
Bela Molnar,
Hermann Lage,
Zoltan Szallasi,
Aron C. Eklund

Published: May 21, 2009
https://doi.org/10.1371/journal.pone.0005645

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Doing the "correct" q-pcr

Posted by sinhaa on 04 Sep 2009 at 15:38 GMT

The choice of normalization gene(s) and method is very important, especially in studies which compare Q-PCR vs. microarray results. The rational is nicely described in this report. I wish more and more scientists adopt similar robust methodologies.
-Amit.

No competing interests declared.

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Preprocessing
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Reverse transcriptase-polymerase chain reaction
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Evaluation of Microarray Preprocessing Algorithms Based on Concordance with RT-PCR in Clinical Samples

Evaluation of Microarray Preprocessing Algorithms Based on Concordance with RT-PCR in Clinical Samples

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Doing the "correct" q-pcr

Posted by sinhaa on 04 Sep 2009 at 15:38 GMT