Testing the Limits of 454 Pyrotag Sequencing: Reproducibility, Quantitative Assessment and Comparison to T-RFLP Fingerprinting of Aquifer Microbes

Open Access

Peer-reviewed

Research Article

Giovanni Pilloni,

Affiliation Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany
⨯
Michael S. Granitsiotis,

Affiliation Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany
⨯
Marion Engel,

Affiliation Research Unit Environmental Genomics, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany
⨯
Tillmann Lueders

* E-mail: tillmann.lueders@helmholtz-muenchen.de

Affiliation Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany
⨯

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DNA replication
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Community structure
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Polymerase chain reaction
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Ribosomal RNA
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Genetic fingerprinting
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DNA extraction
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Libraries
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