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DMEM: KSFM ratio in USC differentiation

Posted by BanKurdi on 12 Apr 2014 at 11:41 GMT

In the protocol you utilized to assess the ability of USC to differentiate towards the urothelial lineage you used DMEM and KSFM in a 4:1 ratio. However, in the article you refereed for this procedure entitled "Characterization of urine-derived stem cells obtained from the upper urinary tract for used in cell based urological tissue engineering" they used DMEM and KSFM in a 1:4 ratio. Is this a typo or is there a reasoning behind your choice.

No competing interests declared.