A Fully Automated Robotic System for Microinjection of Zebrafish Embryos

Wenhui Wang; Xinyu Liu; Danielle Gelinas; Brian Ciruna; Yu Sun

doi:10.1371/journal.pone.0000862

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Open Access

Peer-reviewed

Research Article

A Fully Automated Robotic System for Microinjection of Zebrafish Embryos

Wenhui Wang ,

Contributed equally to this work with: Wenhui Wang, Xinyu Liu

Affiliation Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Canada
⨯
Xinyu Liu ,

Contributed equally to this work with: Wenhui Wang, Xinyu Liu

Affiliation Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Canada
⨯
Danielle Gelinas,

Affiliation Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Canada
⨯
Brian Ciruna ,

*To whom correspondence should be addressed. E-mail: ciruna@sickkids.ca (BC); sun@mie.utoronto.ca (YS)

Affiliation Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, Canada
⨯
Yu Sun

*To whom correspondence should be addressed. E-mail: ciruna@sickkids.ca (BC); sun@mie.utoronto.ca (YS)

Affiliation Advanced Micro and Nanosystems Laboratory, University of Toronto, Toronto, Canada
⨯

A Fully Automated Robotic System for Microinjection of Zebrafish Embryos

Wenhui Wang,
Xinyu Liu,
Danielle Gelinas,
Brian Ciruna,
Yu Sun

Published: September 12, 2007
https://doi.org/10.1371/journal.pone.0000862

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Five stars!

Posted by rnoche on 14 Sep 2007 at 15:46 GMT

In this very impressive paper, Wang et al. developed an excellent and novel automated microrobot injection system for zebrafish (and other) embryos. This is a very significant advance in the delivery of substances (e.g., DNA, RNA, morpholinos) into zebrafish embryos and is amenable to high-throughput studies.

A very minor issue-I am curious about the data on the survival rate. From time to time, there are a handful of infertile zebrafish eggs in a clutch. In all of the experiments in this paper, were infertile eggs excluded, if any?

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A Fully Automated Robotic System for Microinjection of Zebrafish Embryos

A Fully Automated Robotic System for Microinjection of Zebrafish Embryos

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Five stars!

Posted by rnoche on 14 Sep 2007 at 15:46 GMT