Reader Comments
Post a new comment on this article
Post Your Discussion Comment
Please follow our guidelines for comments and review our competing interests policy. Comments that do not conform to our guidelines will be promptly removed and the user account disabled. The following must be avoided:
- Remarks that could be interpreted as allegations of misconduct
- Unsupported assertions or statements
- Inflammatory or insulting language
Thank You!
Thank you for taking the time to flag this posting; we review flagged postings on a regular basis.
closenpl3-regulated genes
Posted by Beschwartz on 13 Oct 2008 at 02:06 GMT
Does the 30% that require npl3 fall into any particular class? In general, why do you need different mechanisms/models for termination?
RE: npl3-regulated genes
mbucheli replied to Beschwartz on 15 Oct 2008 at 03:10 GMT
The 30% of genes affected in the npl3-S411A mutant include some groups of genes that are involved in a common pathway (e.g., ADE, ARP, PHO etc), and also proteins related to a particular activity; for example there is a good number of genes encoding mitochondrial ribosomal proteins (MRLPs, MRPs) or splicing factors (PRPs). But not all members of a common pathway or activity share the same termination phenotype effect.
In answer to the second question, having different mechanisms of termination allows the cells to be more adaptable, or to have potentially higher survival. For instance, in response to a particular stimulus, a given set of genes could be similarly regulated. This already happens at the level of initiation, but perhaps there is coordinated regulation that includes termination. Having different mechanisms of transcription termination would also provide an extra layer of regulation to shut off production of a given mRNA at the termination level without affecting the transcription of all other RNAs.