Reader Comments
Post a new comment on this article
Post Your Discussion Comment
Please follow our guidelines for comments and review our competing interests policy. Comments that do not conform to our guidelines will be promptly removed and the user account disabled. The following must be avoided:
- Remarks that could be interpreted as allegations of misconduct
- Unsupported assertions or statements
- Inflammatory or insulting language
Thank You!
Thank you for taking the time to flag this posting; we review flagged postings on a regular basis.
closescaling in figure 2.
Posted by SteffenDietzel on 06 Nov 2013 at 16:00 GMT
I was wondering about the scaling in Figure 2. According to the legend the scale bar is 100 µm. It has the same length than 3x the distance between the large graduation marks on the top of each image. This means that the distance between two such marks would be 33.3 µm and between small graduation marks 6.66 µm. Although quite possible, this is a rather unusual scale. Therefore it would be nice if the authors could confirm that this is indeed the case.
RE: scaling in figure 2.
CMSTCROIX replied to SteffenDietzel on 11 Nov 2013 at 17:48 GMT
The scale bar more accurately reflects the measurements we obtained with this data set. The scale bar was generated with NIS Elements using the original image acquisition parameters (objective, NA, zoom etc), whereas the graduation marks are assigned by Imaris and are more arbitrary and dependent upon the zoom factor used when viewing the image.