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closeNice data
Posted by karimhnia on 01 Sep 2012 at 11:45 GMT
Dear authors,
This is very nice data showing that DAPC are not statics complexes and I think that this paper will encourage biochemist to study differences between DAPC in several tissues to explain some aspect of DMD physiopathogy.
I have a question about nNOS:
How do authors explain that nNOS do not interact with dystrophin in cardiac muscle, while the 16-17 SR domain exist is it possible that there is only nNOS beta isoform (Golgi) in cardiac muscle but not nNOSμ or there is other cardiac nNOS isoform that do not recognize dystrophin?
Thanks
Best
Karim
RE: Nice data
montanarof replied to karimhnia on 14 Sep 2012 at 20:05 GMT
Dear Karim,
Thank you for your compliments on our data.
You posed an excellent question about nNOS. Unfortunately, we currently do not have an explanation for the lack of association of nNOS to full length cardiac dystrophin.
nNOSmu has been reported to be expressed in the heart (Silvagno et al, JBC, 1996) although its subcellular localization was not examined. Other possibilities to be considered are that:
1) nNOS and the DAPC may be targeted to two different cellular compartments and therefore cannot interact in the heart, or
2) something else may be masking one of the nNOS binding sites on dystrophin or syntrophin alpha1. We did find differences in the syntrophin and dystrobrevin composition of the cardiac DAPC that may affect the affinity of the dystrophin-nNOS association.
How different tissues regulate the inclusion and exclusion of specific proteins from the DAPC will definitely be an important area of future investigation.
Best,
Federica