Protein-Tyrosine Kinase Activity Profiling in Knock Down Zebrafish Embryos

Simone Lemeer; Chris Jopling; Faris Naji; Rob Ruijtenbeek; Monique Slijper; Albert J.R. Heck; Jeroen den Hertog

doi:10.1371/journal.pone.0000581

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Open Access

Peer-reviewed

Research Article

Protein-Tyrosine Kinase Activity Profiling in Knock Down Zebrafish Embryos

Simone Lemeer ,

Contributed equally to this work with: Simone Lemeer, Chris Jopling

Affiliations Hubrecht Institute, Utrecht, The Netherlands, Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
⨯
Chris Jopling ,

Contributed equally to this work with: Simone Lemeer, Chris Jopling

Affiliation Hubrecht Institute, Utrecht, The Netherlands
⨯
Faris Naji,

Affiliation Pamgene International B.V., Hertogenbosch, The Netherlands
⨯
Rob Ruijtenbeek,

Affiliation Pamgene International B.V., Hertogenbosch, The Netherlands
⨯
Monique Slijper,

Affiliation Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
⨯
Albert J.R. Heck,

Affiliation Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
⨯
Jeroen den Hertog

*To whom correspondence should be addressed. E-mail: hertog@niob.knaw.nl

Affiliation Hubrecht Institute, Utrecht, The Netherlands
⨯

Protein-Tyrosine Kinase Activity Profiling in Knock Down Zebrafish Embryos

Simone Lemeer,
Chris Jopling,
Faris Naji,
Rob Ruijtenbeek,
Monique Slijper,
Albert J.R. Heck,
Jeroen den Hertog

Published: July 4, 2007
https://doi.org/10.1371/journal.pone.0000581

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Excellent!

Posted by JonMoulton on 25 Jul 2007 at 17:42 GMT

This paper describes a new technique for rapidly profiling the kinase activity of a cell. Using Morpholino antisense the authors individually knocked down the expression of targeted kinases and observed the changes in the global kinase activity profile of the cell. This work combined the specificity of Morpholino antisense with PanGene's peptide array assay system to show a new path to high-throughput assays of enzyme activity and characterization of the contributions of individual protein to those enzyme activity profiles.

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Phosphorylation
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Embryos
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Zebrafish
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Signal peptides
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Protein kinases
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Wnt signaling cascade
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Reaction kinetics
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Protein-Tyrosine Kinase Activity Profiling in Knock Down Zebrafish Embryos

Protein-Tyrosine Kinase Activity Profiling in Knock Down Zebrafish Embryos

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Posted by JonMoulton on 25 Jul 2007 at 17:42 GMT