Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)

Jonathan E. Foley; Jing-Ruey J. Yeh; Morgan L. Maeder; Deepak Reyon; Jeffry D. Sander; Randall T. Peterson; J. Keith Joung

doi:10.1371/journal.pone.0004348

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Open Access

Peer-reviewed

Research Article

Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)

Jonathan E. Foley ,

Contributed equally to this work with: Jonathan E. Foley, Jing-Ruey J. Yeh

Affiliation Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America
⨯
Jing-Ruey J. Yeh ,

Contributed equally to this work with: Jonathan E. Foley, Jing-Ruey J. Yeh

Affiliations Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, United States of America
⨯
Morgan L. Maeder,

Affiliation Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America
⨯
Deepak Reyon,

Affiliation Department of Genetics, Development and Cell Biology, Interdepartmental Graduate Program in Bioinformatics and Computational Biology, Iowa State University, Ames, Iowa, United States of America
⨯
Jeffry D. Sander,

Affiliation Department of Genetics, Development and Cell Biology, Interdepartmental Graduate Program in Bioinformatics and Computational Biology, Iowa State University, Ames, Iowa, United States of America
⨯
Randall T. Peterson,

Affiliations Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts, United States of America, Broad Institute of MIT and Harvard, Cambridge, Massachusetts, United States of America
⨯
J. Keith Joung

* E-mail: jjoung@partners.org

Affiliations Molecular Pathology Unit, Center for Cancer Research, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Charlestown, Massachusetts, United States of America, Department of Pathology, Harvard Medical School, Boston, Massachusetts, United States of America
⨯

Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)

Jonathan E. Foley,
Jing-Ruey J. Yeh,
Morgan L. Maeder,
Deepak Reyon,
Jeffry D. Sander,
Randall T. Peterson,
J. Keith Joung

Published: February 9, 2009
https://doi.org/10.1371/journal.pone.0004348

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Minor correction

Posted by Joung on 26 Feb 2010 at 03:09 GMT

[26]
http://plosone.org/article/info:doi/10.1371/journal.pone.0004348#article1.body1.sec4.sec6.p1

Currently available OPEN reagents include not only those in ref. 26 (Maeder et al, 2008) but also pools for F1 AAC, F1 TGC, F1 TTG, and F3 TGT.

No competing interests declared.

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Zebrafish
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Zinc finger nucleases
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Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)

Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)

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Minor correction

Posted by Joung on 26 Feb 2010 at 03:09 GMT