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Determination of collagen afifnity of peptide is unclear

Posted by mmerkx on 28 Oct 2013 at 07:49 GMT

The authors clearly show that the cyclic peptide collagelin has some afifnity for binding to collagen. The dissociation constant for binding to collagen is reported to be 0.1 microM based on the SPR experiments shown in Fig 2B. However, this figure shows only two curves representing association and dissociation at 250 microg/mL (= 100 microM) and 500 microg/mL (=200 microM). These are the only 2 peptide concentrations that are reported, and since both them are 1000-fold higher than the reported Kd it is unclear how the reported Kd could be derived from these data. The increase in binding response between 100 microM and 200 microM suggests that the Kd is actually much higher, corresponding to weaker binding.

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