Immunization with Cocktail of HIV-Derived Peptides in Montanide ISA-51 Is Immunogenic, but Causes Sterile Abscesses and Unacceptable Reactogenicity

Barney S. Graham; M. Juliana McElrath; Michael C. Keefer; Kyle Rybczyk; David Berger; Kent J. Weinhold; Janet Ottinger; Guido Ferarri; David C. Montefiori; Don Stablein; Carol Smith; Richard Ginsberg; John Eldridge; Ann Duerr; Pat Fast; Barton F. Haynes; the AIDS Vaccine Evaluation Group

doi:10.1371/journal.pone.0011995

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Open Access

Peer-reviewed

Research Article

Immunization with Cocktail of HIV-Derived Peptides in Montanide ISA-51 Is Immunogenic, but Causes Sterile Abscesses and Unacceptable Reactogenicity

Barney S. Graham ,

* E-mail: bgraham@nih.gov

Affiliation Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America
⨯
M. Juliana McElrath,

Affiliation Fred Hutchinson Cancer Research Center, University of Washington at Seattle, Seattle, Washington, United States of America
⨯
Michael C. Keefer,

Affiliation University of Rochester School of Medicine and Dentistry, Rochester, New York, United States of America
⨯
Kyle Rybczyk,

Affiliation Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America
⨯
David Berger,

Affiliation Fred Hutchinson Cancer Research Center, University of Washington at Seattle, Seattle, Washington, United States of America
⨯
Kent J. Weinhold,

Affiliation Duke University School of Medicine, Durham, North Carolina, United States of America
⨯
Janet Ottinger,

Affiliation Duke University School of Medicine, Durham, North Carolina, United States of America
⨯
Guido Ferarri,

Affiliation Duke University School of Medicine, Durham, North Carolina, United States of America
⨯
David C. Montefiori,

Affiliation Duke University School of Medicine, Durham, North Carolina, United States of America
⨯
Don Stablein,

Affiliation EMMES Corporation, Rockville, Maryland, United States of America
⨯
Carol Smith,

Affiliation EMMES Corporation, Rockville, Maryland, United States of America
⨯
Richard Ginsberg †,

† Deceased.
⨯
John Eldridge,

Affiliation Profectus Biosciences, Inc., Tarrytown, New York, United States of America
⨯
Ann Duerr,

Affiliation Fred Hutchinson Cancer Research Center, University of Washington at Seattle, Seattle, Washington, United States of America
⨯
Pat Fast,

Affiliation International AIDS Vaccine Initiative, New York, New York, United States of America
⨯
Barton F. Haynes,

Affiliation Duke University School of Medicine, Durham, North Carolina, United States of America
⨯
[ ... ],
the AIDS Vaccine Evaluation Group
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Immunization with Cocktail of HIV-Derived Peptides in Montanide ISA-51 Is Immunogenic, but Causes Sterile Abscesses and Unacceptable Reactogenicity

Barney S. Graham,
M. Juliana McElrath,
Michael C. Keefer,
Kyle Rybczyk,
David Berger,
Kent J. Weinhold,
Janet Ottinger,
Guido Ferarri,
David C. Montefiori,
Don Stablein

Published: August 10, 2010
https://doi.org/10.1371/journal.pone.0011995

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Diminishing local reactions caused by oily ISA-51

Posted by huangminghsi on 12 Aug 2010 at 04:25 GMT

To diminish local reactions caused by oily ISA-51, one can use hydrophilic emulsifiers such as Tween or PEG-based bioresorbable polymers to stabilize the interfaces between the oily Montanide ISA 51 adjuvant and the antigen media. Tween or polymer-stabilized ISA51 emulsions have high affinity to water, such that the stock of antigen-encapsulating emulsion could be re-dispersed into PBS before injection, thus yielding stable and injectable W/O/W emulsion nanoparticles, as described in Pharm Res 2009, 26(8), 1856. doi: 10.1007/s11095-009-9898-y

No competing interests declared.

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Immunization with Cocktail of HIV-Derived Peptides in Montanide ISA-51 Is Immunogenic, but Causes Sterile Abscesses and Unacceptable Reactogenicity

Immunization with Cocktail of HIV-Derived Peptides in Montanide ISA-51 Is Immunogenic, but Causes Sterile Abscesses and Unacceptable Reactogenicity

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Diminishing local reactions caused by oily ISA-51

Posted by huangminghsi on 12 Aug 2010 at 04:25 GMT