Testing the Limits of 454 Pyrotag Sequencing: Reproducibility, Quantitative Assessment and Comparison to T-RFLP Fingerprinting of Aquifer Microbes

• Giovanni Pilloni,

  Affiliation Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany

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• Michael S. Granitsiotis,

  Affiliation Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany

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• Marion Engel,

  Affiliation Research Unit Environmental Genomics, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany

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• Tillmann Lueders

  * E-mail: tillmann.lueders@helmholtz-muenchen.de

  Affiliation Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany

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