The Yeast Tor Signaling Pathway Is Involved in G2/M Transition via Polo-Kinase

Akio Nakashima; Yoshiko Maruki; Yuko Imamura; Chika Kondo; Tomoko Kawamata; Ippei Kawanishi; Hideki Takata; Akira Matsuura; Kyung S. Lee; Ushio Kikkawa; Yoshinori Ohsumi; Kazuyoshi Yonezawa; Yoshiaki Kamada

doi:10.1371/journal.pone.0002223

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Open Access

Peer-reviewed

Research Article

The Yeast Tor Signaling Pathway Is Involved in G2/M Transition via Polo-Kinase

Akio Nakashima,

Current address: Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, California, United States of America

Affiliations Biosignal Research Center, Kobe University, Kobe, Japan, CREST, Japan Science and Technology Agency, Kawaguchi, Japan
⨯
Yoshiko Maruki,

Affiliation Biosignal Research Center, Kobe University, Kobe, Japan
⨯
Yuko Imamura,

Affiliation Division of Molecular Cell Biology, National Institute for Basic Biology, Okazaki, Japan
⨯
Chika Kondo,

Affiliation Division of Molecular Cell Biology, National Institute for Basic Biology, Okazaki, Japan
⨯
Tomoko Kawamata,

Affiliation Division of Molecular Cell Biology, National Institute for Basic Biology, Okazaki, Japan
⨯
Ippei Kawanishi,

Affiliation Biosignal Research Center, Kobe University, Kobe, Japan
⨯
Hideki Takata,

Affiliation Department of Geriatric Research, National Institute for Longevity Sciences, Obu Aichi, Japan
⨯
Akira Matsuura,

Current address: Graduate School of Advanced Integration Science, Chiba University, Chiba, Japan

Affiliation Department of Geriatric Research, National Institute for Longevity Sciences, Obu Aichi, Japan
⨯
Kyung S. Lee,

Affiliation Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
⨯
Ushio Kikkawa,

Affiliation Biosignal Research Center, Kobe University, Kobe, Japan
⨯
Yoshinori Ohsumi,

Affiliation Division of Molecular Cell Biology, National Institute for Basic Biology, Okazaki, Japan
⨯
Kazuyoshi Yonezawa,

Affiliations Biosignal Research Center, Kobe University, Kobe, Japan, CREST, Japan Science and Technology Agency, Kawaguchi, Japan
⨯
Yoshiaki Kamada

* E-mail: yoshikam@nibb.ac.jp

Affiliations CREST, Japan Science and Technology Agency, Kawaguchi, Japan, Division of Molecular Cell Biology, National Institute for Basic Biology, Okazaki, Japan
⨯

The Yeast Tor Signaling Pathway Is Involved in G2/M Transition via Polo-Kinase

Akio Nakashima,
Yoshiko Maruki,
Yuko Imamura,
Chika Kondo,
Tomoko Kawamata,
Ippei Kawanishi,
Hideki Takata,
Akira Matsuura,
Kyung S. Lee,
Ushio Kikkawa

Published: May 21, 2008
https://doi.org/10.1371/journal.pone.0002223

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Comments from Faculty of 1000 Biology

Posted by NiyazAhmed on 29 Jun 2008 at 05:09 GMT

Evaluated by: Joe Heitman with Cecelia Shertz and Maria E. Cardenas, Duke University Medical Center, United States of America [MICROBIOLOGY]
Evaluated 23 Jun 2008

Tags: Hypothesis, New Finding, Novel Drug Target
F1000 Factor: 3.0

Comments:
The ubiquitous Tor nutrient sensor cascade was discovered to evoke G2/M cell cycle transition via the polo-like kinase Cdc5 in Saccharomyces cerevisiae. It was known that inhibition of Tor with rapamycin causes a G1 cell cycle arrest, whereas these new findings by Nakashima et al. demonstrate that disruption of the Tor complex 1 (TORC1) provokes a G2/M delay. This effect is attributable to TORC1-protein phosphatase 2A mediated Cdc5 nuclear import, which has multiple mitotic roles including Swe1 phosphorylation which controls G2/M transition, cytokinesis, and CLB2 expression. This study reveals Tor is active throughout the cell cycle via key cell cycle regulators. This scenario differs from that found in Schizosaccharomyces pombe, where rapamycin stimulates mitotic entry {1}. Future studies should address if TORC1 governs the G2/M transition in multi-cellular eukaryotes such as humans. Reference: {1} Petersen et al. Nat Cell Biol 2007, 9:1263-1272.

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Best,

Niyaz Ahmed
Section Editor, PLoS ONE

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The Yeast Tor Signaling Pathway Is Involved in G2/M Transition via Polo-Kinase

The Yeast Tor Signaling Pathway Is Involved in G2/M Transition via Polo-Kinase

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Comments from Faculty of 1000 Biology

Posted by NiyazAhmed on 29 Jun 2008 at 05:09 GMT