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Materials and Methods for Magnetic Bead-Ab Conjugation and Anti-body Cross-reactivity

Posted by jwade1221 on 30 Jan 2013 at 16:56 GMT

I am curious about the method for conjugating the antibodies to the magnetic beads for cell lysate enrichment. How were the abs conjugated to the beads, and what methods were used to verify successful conjugation? Additionally, what antibodies were used for all antigens of interest? Were any orthogonal methods used to verify that the antibodies were able to discriminate between the phosphorylated and non-phosphorylated signaling proteins?

No competing interests declared.