Designer Self-Assembling Peptide Nanofiber Scaffolds for Adult Mouse Neural Stem Cell 3-Dimensional Cultures

Fabrizio Gelain; Daniele Bottai; Angleo Vescovi; Shuguang Zhang

doi:10.1371/journal.pone.0000119

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Peer-reviewed

Research Article

Designer Self-Assembling Peptide Nanofiber Scaffolds for Adult Mouse Neural Stem Cell 3-Dimensional Cultures

Fabrizio Gelain,

Affiliations Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, Stem Cell Research Institute, Department of Biological and Technological Research, Fondazione Centro San Raffaele del Monte Tabor, Milan, Italy, Bioscience and Biotechnology Department, University of Milan-Bicocca, Milan, Italy
⨯
Daniele Bottai,

Affiliations Stem Cell Research Institute, Department of Biological and Technological Research, Fondazione Centro San Raffaele del Monte Tabor, Milan, Italy, Department of Medicine, Surgery and Dentistry, Faculty of Medicine, University of Milan, Milan, Italy
⨯
Angleo Vescovi,

Affiliations Stem Cell Research Institute, Department of Biological and Technological Research, Fondazione Centro San Raffaele del Monte Tabor, Milan, Italy, Bioscience and Biotechnology Department, University of Milan-Bicocca, Milan, Italy
⨯
Shuguang Zhang

*To whom correspondence should be addressed. E-mail: shuguang@mit.edu

Affiliation Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
⨯

Designer Self-Assembling Peptide Nanofiber Scaffolds for Adult Mouse Neural Stem Cell 3-Dimensional Cultures

Fabrizio Gelain,
Daniele Bottai,
Angleo Vescovi,
Shuguang Zhang

Published: December 27, 2006
https://doi.org/10.1371/journal.pone.0000119

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Artificial Amino Acid Matrices

Posted by Machiavelli on 31 Dec 2006 at 20:15 GMT

I know the science isnt quite there yet which would allow for the inclusion of multiple artificial amino acids (right now Schultz and company have only really played around with unused stop codons). But when the technology becomes available and we can engineer in multiple artificial amino acids into a single system, would this allow us to create better 3D scaffolds?

Additionally would it be possible to engineer tRNA synthethases with the capability of using amino acids with two side chains instead of one (replacement of the hydrogen bound to the chiral carbon with a functional group such as a ketone)?

If it does become possible to use multiple artificial amino acids with secondary functionality, it would seem you could make complex highly ordered 3D nanostructure matrices. Can anyone comment?

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Molecular self assembly
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Neuronal differentiation
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Sequence motif analysis
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Designer Self-Assembling Peptide Nanofiber Scaffolds for Adult Mouse Neural Stem Cell 3-Dimensional Cultures

Designer Self-Assembling Peptide Nanofiber Scaffolds for Adult Mouse Neural Stem Cell 3-Dimensional Cultures

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Artificial Amino Acid Matrices

Posted by Machiavelli on 31 Dec 2006 at 20:15 GMT