Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

Kimberly A. Kelly; Jonathan Carson; Jason R. McCarthy; Ralph Weissleder

doi:10.1371/journal.pone.0000665

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Peer-reviewed

Research Article

Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

Kimberly A. Kelly ,

Contributed equally to this work with: Kimberly A. Kelly, Jonathan Carson

Affiliation Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Jonathan Carson ,

Contributed equally to this work with: Kimberly A. Kelly, Jonathan Carson

Affiliation Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Jason R. McCarthy,

Affiliation Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
⨯
Ralph Weissleder

*To whom correspondence should be addressed. E-mail: weissleder@helix.mgh.harvard.edu

Affiliation Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
⨯

Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

Kimberly A. Kelly,
Jonathan Carson,
Jason R. McCarthy,
Ralph Weissleder

Published: July 25, 2007
https://doi.org/10.1371/journal.pone.0000665

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Another potential use...

Posted by iyudushkin on 14 Sep 2007 at 01:05 GMT

Certain organic dyes, including some widely used rhodamine-, BODIPY- and indocyanine-based fluorophores, sometimes exhibit unspecific sorption on cellular structures when used for live- or fixed-cell imaging. A short peptide that appears to interact with nanomolar affinity with certain fluorophores, such as the one reported in this study, would be an excellent washing reagent to decrease unspecific dye sorption and eliminate the background staining (as demonstrated in a competition expt in Fig. 6A).

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Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

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Another potential use...

Posted by iyudushkin on 14 Sep 2007 at 01:05 GMT