We have seen a few mentions in publication of figure S1, and I've received a few emails about it as well. In early experiments we did see a drop in CFU in all strains. In the last eight years of using this model I typically see less than a two fold drop in CFU as the bacteria enter hypoxia. The results published in the Sherrid et. al 2010 PLoS One paper, Figure 1, are representative of our typical results. Unfortunately, this was early comparison makes the point that we don't see any phenotype in our standard hypoxic model with the DosR mutant, but it is hard to ignore the drop in CFU seen in both strains. Subsequent work has repeated the observation the figure is trying to convey, without the drop in CFU, but it is sadly too late to update the figure. Perhaps in another paper soon....
Tige Rustad
